What is the host and type of this antibody?

This antibody, TAB-011, is a humanized IgG1. It originates from a non-human species but has been engineered with human framework regions to be more compatible with human systems for research. It is recombinantly produced in a CHO (Chinese Hamster Ovary) expression system, which is a standard for producing glycosylated antibodies.

Is this anti-VEGF antibody validated for flow cytometry?

Yes, our internal data confirms that this antibody is validated for use in Flow Cytometry (FC). In addition, it is also validated for a comprehensive range of other common research applications, including Immunoprecipitation (IP), Immunohistochemistry (IHC), and Immunofluorescence (IF).

Anti-Human VEGF Recombinant Antibody (Bevacizumab)

CAT#: TAB-011

Recombinant monoclonal antibody to Human VEGF is an angiogenesis inhibitor, a drug that slows the growth of new blood vessels. It is licensed to treat various cancers, including colorectal, lung, breast (outside the USA), glioblastoma (USA only), kidney and ovarian. Bevacizumab is a humanized monoclonal antibody that produces angiogenesis inhibition by inhibiting vascular endothelial growth factor A (VEGF-A). VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in cancer.

Tested Data Gene Expression

Figure 1 Anti-Human VEGF Recombinant Antibody (TAB-011) in WB

Western blot analysis of TAB-011 was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-011 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 2 Anti-Human VEGF Recombinant Antibody (TAB-011) in WB

Western blot analysis of TAB-011 was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-011 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 3 Anti-Human VEGF Recombinant Antibody (TAB-011) in DB

Dot Blot analysis of TAB-011 was performed by coating with Human VEGF165 Protein (His Tag).

TAB-011 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 4 Anti-Human VEGF Recombinant Antibody (TAB-011) in DB

Dot Blot analysis of TAB-011 was performed by coating with Human VEGF121 Protein (His Tag).

TAB-011 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

ELISA

Figure 5 Anti-Human VEGF Recombinant Antibody (TAB-011) in ELISA

ELISA analysis of TAB-011 was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with TAB-011. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

ELISA

Figure 6 Anti-Human VEGF Recombinant Antibody (TAB-011) in ELISA

ELISA analysis of TAB-011 was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with TAB-011. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA

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Specifications

Immunogen
Purified intact human VEGF

Host Species
Mouse

Derivation
Humanized (from mouse)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen

Species Reactivity
Human

Applications
Suitable for use in FC, IP, ELISA, Neut, FuncS, IF, ICC and most other immunological methods.

Trade name
Bevacizumab

CAS
216974-75-3

Generic Name
Bevacizumab

Biological Half-Life
20 days (range: 11–50 days)

ATC Code
L01XC07

DrugBank
DB00112

UNII
2S9ZZM9Q9V

ChEMBL
CHEMBL1201583

MW
149 kDa

Related Disease
Colorectal cancers, metastatic

Product Property

Purity
>95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

Alternative Names
Bevacizumab;bevacizumab;216974-75-3;DB00112VEGFA;vascular endothelial growth factor A;vascular endothelial growth factor , VEGF;VEGF A;VPF;vascular permeability factor;VEGF;MVCD1;MGC70609;

Gene ID
7422

UniProt ID
P15692

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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There are currently no Customer reviews or questions for TAB-011. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Versatile VEGF Antibody

We needed an anti-human VEGF antibody for a few different assays. This product (TAB-011) was appealing because it's validated for a wide range, including FC, IF, and ICC. Having a humanized IgG1 antibody expressed in CHO cells gives us confidence in its consistency for our research.

Good for Imaging

Our lab focuses on imaging, so we were happy to find this humanized anti-VEGF antibody is validated for both IHC and IF. This allows us to visualize VEGF expression in our human cell and tissue models. The broad validation for other applications like FC and IP is also a major plus.

Q&As

What is the host and type of this antibody?

A: This antibody, TAB-011, is a humanized IgG1. It originates from a non-human species but has been engineered with human framework regions to be more compatible with human systems for research. It is recombinantly produced in a CHO (Chinese Hamster Ovary) expression system, which is a standard for producing glycosylated antibodies.
Is this anti-VEGF antibody validated for flow cytometry?

A: Yes, our internal data confirms that this antibody is validated for use in Flow Cytometry (FC). In addition, it is also validated for a comprehensive range of other common research applications, including Immunoprecipitation (IP), Immunohistochemistry (IHC), and Immunofluorescence (IF).

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-011, RRID: AB_3111741)

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Publications

Peer-reviewed Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Nouadje, Georges, and Thierry Ligneel. "Improved immunofixation electrophoresis method with target component on-gel immunodisplacement." U.S. Patent Application No. 16/320,521.

Biosimilar Overview

Please refer to Bevacizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Bevacizumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-011.pdf

MSDS

MSDS - TAB-011.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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Afuco™ Anti-VEGFA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-011)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human VEGF is an angiogenesis inhibitor, a drug that slows the growth of new blood vessels. It is a humanized monoclonal antibody that produces angiogenesis inhibition by inhibiting vascular endothelial growth factor A (VEGF-A). VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in cancer.

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